Fig. 7.

Low-fat/high-carbohydrate diet, but not Western diet, upregulates cardiac delta-9 desaturase gene expression. Transcript levels of fatty acid synthase (fasn; A), the long chain fatty acid elongase ELOVL family member 6 (elovl6; B), acetyl-CoA carboxylase 1 (acc1; C), stearoyl-CoA desaturase isoforms 1 and 2 (scd1 and scd2; D and E, respectively), sterol regulatory element-binding proteins 1 (srebp1; F), and carbohydrate response element binding protein (chrebp; G) were quantified by real-time PCR in the heart of Wistar rats fed either low-fat/high-carbohydrate diet (HCD; open bars), high-fat diet (HFD; hatched bars), or Western diet (WD; black bars) for acute term (AT), short term (ST), intermediate term (IT), or long term (LT). Baseline mRNA levels (B; gray bar) were determined from 8-week-old rats fed standard rodent chow for 2 weeks before the beginning of the feeding protocol. Data are means ± SE of n = 13–18 animals per group per time point (except baseline; n = 6). One, two, and three symbols represent P < 0.05, P < 0.01, and P < 0.001, respectively. # = vs. Western diet at same age; $ = vs. high-fat diet at same age; a = vs. baseline; b = vs. acute term; c = vs. short term.