TABLE 1.

Primers used for RT-PCR quantitation of mRNA levels

Species	Gene	Sense Primer	Antisense Primer
Homo sapiens	Srebp-1c	5′-GGAGGGGTAGGGCCAACGGCCT-3′	5′-CATGTCTTCGAAAGTGCAATCC-3′
	Fas	5′-ACAGGGACAACCTGGAGTTCT-3′	5′-CTGT GGTCCCACTTGATGAGT-3′
	Acc1	5′-GTTGCACAAAAGGATTTCAG-3′	5′-CGCATTACCATGCTCCGCAC-3′
	Dgat2	5′-GAATCGTGGATCCCAAA-3′	5′-GTCTTCATATAACCAAAGCGGG-3′
	Srebp-2	5′-CAAGATGCACAAGTCTGGCG -3′	5′-GCTTCAGCACCATGTTCTCCTG-3′
	Apoe	5′-CCCAGGTCACCCAGGAACT-3′	5′-TTCCGATTTGTAGGCCTTCAA-3′
	ApoA-I	5′-ATCGAGTGAAGGACCTGGC-3′	5′-AGCTTGCTGAAGGTGGAGGT-3′
	Cpt1a	5′-TGCTTTACAGGCGCAAACTG-3′	5′-TGGAATCGTGGATCCCAAA-3′
	Gapdh	5′-CCCATCACCATCTTCCAGGAG-3′	5′-CTTCTCCATGGTGGTGAAGACG-3′
Mus musculus	Srebp-1c	5′-ATCGGCGCGGAAGCTGTCGGGGTAGC GTC-3′	5′-ACTGTCTTGGTTGTTGATG AGCTGGAGCAT-3′
	Cpt1a	5′-CGCACGGAAGGAAAATGG-3′	5′-TGTGCCCAATATTCCTGG-3′
	Acc1	5′-ATTGGGCACCCCAGAGCTA-3′	5′-CCCGCTCCTTCAACTTGCT-3′
	Dgat2	5′- ACTCTGGAGGTTGGCACCAT-3′	5′-GGGTGTGGCTCAGGAGGAT-3′
	Fas	5′-CATGACCTCGTGATGAACGTGT-3′	5′-CGGGTGAGGACGTTTACAAAG-3′
	Srebp-2	5′-CCGAGATGCAGGGCAAAG-3′	5′-GATGAAAGAACAATGAACAA GGCTTA-3′
	Gapdh	5′-CCTGCACCACCAACTGCTTA-3′	5′-TCATGAGCCCTTCCACAATG-3′

Abbreviations: ACC1, acyl-CoA carboxylase 1; dn-cJun, dominant negative cJun; Cpt1α, carnitine palmitoyl transferase 1α; DGAT2, diacylglycerol acyltransferase-2; miR, microRNA; SREBP-1c, sterol-regulatory element binding protein 1c; UTR, untranslated region.