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Expression of wild-type and mutant forms of the GPIHBP1 protein in CHO pgsA-745 cells. The cells were electroporated with an empty vector, a construct encoding an S-tagged human GPIHBP1, or the mutants C65S and C68G. GPIHBP1 in nonpermeabilized (A) and permeabilized (B) cells was assessed by immunofluorescence microscopy with an antiserum against the S-protein tag (green). Cell nuclei were visualized with 4’,6-diamidino-2-phenylindole (blue).
