TABLE 2.

Variation, recovery, limit of detection (LOD), limit of quantification (LOQ) and regression data from dried blood spot material (level 1: 5 µmol/L, level 2: 20 µmol/L bile acid)

		Intra-assay	Inter-assay	Recovery [%]	LOQ	LOD	Linear Regression Parameters
Name	Level	[CV in %] n = 10			[nmol/L]		Slope	Intercept	Regression Coefficient
TDC	1	7.4	9.9	63.3	14.0	4.7	0.8378	0.0210	0.9983
	2	6.0	8.1	65.7
DC	1	9.4	11.0	67.7	83.0	27.7	0.1791	0.0750	0.9977
	2	7.8	8.8	68.1
TC	1	7.4	9.2	62.8	19.0	6.3	0.0882	0.0741	0.9992
	2	7.4	8.9	67.8
GCDC	1	7.4	10.2	70.9	21.0	7.0	0.1082	0.0277	0.9979
	2	6.1	7.9	69.2
CDC	1	7.2	8.7	65.2	91.0	30.3	0.1403	0.0232	0.9989
	2	6.9	8.0	64.4
GDC	1	6.2	8.2	67.4	23.0	7.7	0.1143	0.0289	0.9966
	2	8.0	9.6	71.8
TLC	1	3.7	10.2	67.1	11.0	3.7	0.1563	0.2604	0.9956
	2	6.2	9.7	65.8
GC	1	3.9	7.1	66.8	26.0	8.7	0.0822	0.0348	0.9986
	2	6.2	7.7	68.6
LC	1	11.1	12.0	59.5	14.0	4.7	0.1335	0.8212	0.9991
	2	10.2	12.3	58.9
UDC	1	7.1	9.2	62.9	39.0	13.0	0.3015	0.1552	0.9965
	2	6.4	11.1	64.9
TUDC	1	4.7	12.6	60.0	14.0	4.7	0.6840	0.5813	0.9961
	2	7.3	13.0	68.7
TCDC	1	6.1	9.5	63.7	11.0	3.7	0.8343	0.2117	0.9969
	2	7.1	8.8	61.1
GUDC	1	7.2	8.7	62.5	18.0	6.0	0.1220	0.0156	0.9982
	2	7.5	9.0	64.4
GLC	1	5.1	7.3	68.9	45.0	15.0	0.0974	0.0200	0.9977
	2	7.8	8.3	71.6
CA	1	6.8	9.6	66.9	78.0	26.0	0.1672	0.1005	0.9977
	2	7.6	9.1	69.6
DHCA	1	5.8	8.4	59.0	11.0	3.7	0.7233	0.1156	0.9989
	2	7.2	9.4	76.0
THCA	1	8.8	8.4	60.0	21.0	7.0	0.6267	0.1024	0.9965
	2	7.6	10.1	69.0