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. 2010 Jun;51(6):1599–1609. doi: 10.1194/jlr.D004077

Fig. 3.

Fig. 3.

Product-ion analyses of sulfatide species in the negative ion refl ector mode using 9-AA as matrix. Tandem mass spectra of d18:1-N24:1 sulfatide at m/z 888.67 (A) and d18:1-[OH]N24:0 sulfatide at m/z 906.67 (B) present in mouse brain cortical lipid extracts acquired by MALDI-TOF/TOF-MS in the negative ion mode as described in “Materials and Methods.” Each tandem mass spectrum was recorded on a 4800 MALDI-TOF/TOF Analyzer in the negative ion refl ector mode using 9-AA as matrix using collision-induced dissociation with the metastable suppressor on and the timed ion selector enabled. The voltages of source 1, collision cell, and collision cell offset were 8.0, 7.0, and −0.035 kV, respectively. The tandem mass spectrum was obtained by averaging 3000 consecutive laser shots (50 shots/subspectra with 60 total subspectra).