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. 2010 Jun;51(6):1599–1609. doi: 10.1194/jlr.D004077

Fig. 6.

Fig. 6.

MALDI-MS mass spectra of sulfatides in lipid extracts of mouse spinal cord, kidney medulla, kidney cortex, and skeletal muscle. Total lipids of spinal cord, kidney medulla, kidney cortex, and skeletal muscle of mice at 4–6 months of age were extracted by using a modifi ed procedure of Bligh and Dyer against 50 mM LiCl as previously described (39). Part of the lipid extract of mouse kidney medulla, kidney cortex, or skeletal muscle was treated with lithium methoxide as previously described (27). MALDI mass spectra of lipid extracts of mouse spinal cord (A), kidney medulla (B), kidney cortex (C), and skeletal muscle (D) were acquired by averaging 3,000 consecutive laser shots (50 shots/subspectra with 60 total subspectra) using the 4800 MALDI TOF/TOF Analyzer with 9-AA as matrix. IS, Internal standard; ST, sulfatide.