TABLE 1.
Quantitative analysis of sulfatide species in the lipid extracts from a variety of mouse tissue samples and plasmaa
| Molecular species | m/z | Plasmab | Liverb | Muscleb | Kidney cortexb | Kidney medullab | Cortexc | Spinal cordc | Sciatic nervec |
|---|---|---|---|---|---|---|---|---|---|
| d18:0-N14:0 | 752.50 | 8.8 ± 0.7 | 22.4 ± 3.0 | ||||||
| d18:1-N16:1 | 776.50 | 63.3 ± 1.6 | 117.9 ± 14.5 | ||||||
| d18:1-N16:0 | 778.51 | 101.5 ± 1.2 | 282.5 ± 27.8 | ||||||
| d18:0-N16:0 | 780.53 | 7.5 ± 0.2 | 19.2 ± 2.1 | 0.5 ± 0.0 | 2.1 ± 0.1 | 2.8 ± 0.1 | |||
| d18:1-OHN16:1 | 792.49 | 0.5 ± 0.1 | 0.4 ± 0.1 | 3.6 ± 0.4 | 9.7 ± 1.9 | 0.1 ± 0.0 | 0.23 ± 0.02 | 0.3 ± 0.0 | |
| d18:1-OHN16:0 | 794.51 | 0.2 ± 0.0 | 0.9 ± 0.2 | 7.8 ± 1.1 | 50.7 ± 4.7 | 0.4 ± 0.0 | 1.4 ± 0.1 | 1.2 ± 0.1 | |
| d18:0-OHN16:0 | 796.52 | 0.1 ± 0.0 | 1.6 ± 0.1 | 2.7 ± 0.1 | 0.2 ± 0.0 | 0.3 ± 0.0 | |||
| d18:1-N18:1 | 804.53 | 2.4 ± 0.3 | 0.1 ± 0.1 | 0.1 ± 0.0 | 0.3 ± 0.0 | ||||
| d18:1-N18:0 | 806.55 | 1.0 ± 0.4 | 0.2 ± 0.1 | 1.2 ± 0.2 | 0.7 ± 0.1 | 3.5 ± 0.1 | 0.4 ± 0.0 | 1.7 ± 0.2 | 2.9 ± 0.1 |
| d18:0-N18:0 | 808.56 | 0.2 ± 0.0 | 0.5 ± 0.0 | 2.6 ± 0.1 | 0.1 ± 0.0 | 0.3 ± 0.0 | 0.9 ± 0.0 | ||
| d18:1-OHN18:1 | 820.52 | 0.2 ± 0.1 | 2.1 ± 0.2 | 5.1 ± 0.2 | 0.1 ± 0.0 | 0.1 ± 0.0 | |||
| d18:1-OHN18:0 | 822.54 | 0.2 ± 0.0 | 1.2 ± 0.1 | 11.7 ± 0.3 | 0.2 ± 0.0 | 0.4 ± 0.0 | 0.5 ± 0.0 | ||
| d18:1-N20:0 | 834.58 | 0.6 ± 0.1 | 3.6 ± 0.5 | 44.5 ± 2.0 | 0.1 ± 0.0 | 0.7 ± 0.1 | 2.9 ± 0.1 | ||
| d18:0-N20:0 | 836.59 | 0.2 ± 0.0 | 4.2 ± 0.1 | 19.5 ± 1.9 | 0.2 ± 0.1 | 1.1 ± 0.1 | |||
| d18:1-OHN20:1 | 848.56 | 1.6 ± 0.4 | 0.1 ± 0.0 | 16.3 ± 0.9 | 40.5 ± 2.3 | 0.3 ± 0.0 | |||
| d18:1-OHN20:0 | 850.57 | 0.3 ± 0.1 | 0.5 ± 0.1 | 35.9 ± 3.2 | 433.7 ± 39.4 | 0.1 ± 0.0 | 0.5 ± 0.1 | 1.6 ± 0.1 | |
| d18:0-OHN20:0 | 852.59 | 2.6 ± 0.2 | 29.7 ± 2.2 | 0.1 ± 0.0 | 0.7 ± 0.0 | ||||
| d18:1-N22:1 | 860.59 | 9.7 ± 0.2 | 24.2 ± 2.5 | 0.1 ± 0.0 | 0.9 ± 0.1 | 1.1 ± 0.1 | |||
| d18:1-N22:0 | 862.61 | 0.4 ± 0.1 | 0.4 ± 0.0 | 1.9 ± 0.3 | 15.8 ± 2.1 | 195.4 ± 4.3 | 0.7 ± 0.1 | 4.8 ± 0.4 | 9.0 ± 0.5 |
| d18:0-N22:0 | 864.62 | 0.7 ± 0.1 | 17.4 ± 1.0 | 142.3 ± 6.3 | 0.2 ± 0.0 | 1.8 ± 0.2 | 3.5 ± 0.2 | ||
| d18:1-OHN22:2 | 874.57 | 2.0 ± 0.5 | 6.3 ± 0.1 | 14.4 ± 3.2 | |||||
| d18:1-N23:0 /d18:1-OHN22:1 | 876.61 | 1.2 ± 0.3 | 0.1 ± 0.0 | 1.0 ± 0.2 | 16.6 ± 1.4 | 136.8 ± 3.5 | 0.4 ± 0.0 | 1.6 ± 0.1 | 2.2 ± 0.1 |
| d18:1-OHN22:0 | 878.60 | 1.6 ± 0.1 | 0.2 ± 0.0 | 2.8 ± 0.6 | 151.5 ± 16.1 | 2285.7 ± 224.6 | 1.8 ± 0.1 | 6.3 ± 0.4 | 8.5 ± 0.4 |
| d18:0-OHN22:0 | 880.62 | 0.8 ± 0.1 | 14.6 ± 1.3 | 215.5 ± 27.1 | 0.3 ± 0.0 | 2.1 ± 0.2 | 4.4 ± 0.3 | ||
| d18:1-N24:2 | 886.61 | 0.7 ± 0.1 | 0.8 ± 0.1 | 26.7 ± 1.2 | 61.0 ± 5.0 | 0.2 ± 0.0 | 1.0 ± 0.2 | 4.3 ± 0.4 | |
| d18:1-N24:1 | 888.62 | 2.2 ± 0.2 | 0.9 ± 0.1 | 6.2 ± 1.1 | 83.8 ± 2.4 | 202.4 ± 16.0 | 4.0 ± 0.3 | 24.6 ± 2.0 | 27.3 ± 2.3 |
| d18:1-N24:0 /d18:1-OHN23:1 | 890.64 | 0.4 ± 0.1 | 0.7 ± 0.1 | 11.8 ± 2.5 | 55.8 ± 4.7 | 339.3 ± 7.0 | 2.6 ± 0.3 | 17.5 ± 2.0 | 40.8 ± 2.9 |
| d18:0-N24:0 /d18:1-OHN23:0 | 892.65 | 0.3 ± 0.1 | 0.1 ± 0.0 | 3.0 ± 0.6 | 83.5 ± 13.1 | 1337.5 ± 86.4 | 1.0 ± 0.1 | 4.7 ± 0.5 | 9.3 ± 0.7 |
| d18:1-N25:1 /d18:1-OHN24:2 | 902.62 | 5.6 ± 0.5 | 21.3 ± 2.4 | 0.1 ± 0.0 | 0.4 ± 0.0 | 0.6 ± 0.0 | |||
| d18:1-N25:0 /d18:1-OHN24:1 | 904.62 | 1.1 ± 0.2 | 18.9 ± 2.8 | 144.6 ± 2.8 | 0.9 ± 0.1 | 3.1 ± 0.2 | 3.3 ± 0.3 | ||
| d18:1-OHN24:0 | 906.63 | 0.8 ± 0.2 | 0.2 ± 0.0 | 6.8 ± 1.6 | 99.6 ± 15.8 | 1751.3 ± 140.2 | 2.3 ± 0.2 | 8.6 ± 0.6 | 15.3 ± 1.2 |
| d18:0-OHN24:0 | 908.65 | 1.1 ± 0.3 | 12.8 ± 1.3 | 166.3 ± 13.5 | 0.2 ± 0.0 | 1.5 ± 0.2 | 3.9 ± 0.3 | ||
| d18:1-N26:1 | 916.65 | 0.6 ± 0.1 | |||||||
| d18:1-N26:0 /d18:1-OHN25:1 | 918.67 | 66.5 ± 16.0 | 0.6 ± 0.0 | ||||||
| d18:0-N26:0 /d18:1-OHN25:0 | 920.69 | 76.6 ± 3.8 | 0.4 ± 0.0 | ||||||
| Total | 15.5 ± 2.3 | 3.9 ± 0.5 | 42.3 ± 8.6 | 879.3 ± 58.1 | 8310.3 ± 524.3 | 16.5 ± 1.3 | 86.6 ± 5.8 | 151.1 ± 9.5 |
a
Total lipids of each biological sample were extracted by using a modified Bligh and Dyer method as described in Materials and Methods. Negative-ion MALDI-MS analyses of sulfatide species in the lipid extracts were performed using 9-AA as matrix and quantitation by ratiometric comparison of the intensity with that of one or two internal standards was obtained as described in “Materials and Methods.” Data represent the means ± SEM of at least six separate animals.
b
Data are in pmol/mg of protein.
c
Data are in nmol/mg of protein.