Fig. 1.

Electrophoretic scans of HDL₂ and HDL₃ (upper images) isolated from a pool of plasma by ultracentrifugation, further separated by PAGE, and enzymatically stained for cholesterol (left). The results obtained with the enzymatic mixture in buffer without any viscosant agent, with Nobel agar (NA) and with carboxymethylcellulose (CMC) are shown from left to right. HDL₂ and HDL₃ enzymatically stained using CMC are also shown after Coomassie blue staining (right), including the globular proteins used as diameter standards (DS). Densitograms for HDL₂ and HDL₃ (lower graphs) correspond to the enzymatic mixture in buffer (solid black line), using Noble agar (dotted line), CMC (dashed line), and stained with Coomassie blue (solid gray line). For enzymatic staining, samples were incubated 1 h at 37°C. OD, optical density.