Table 2. A list of 30 high frequency novel candidate miRNAs in breast cancer cells.
| NAME | SEQUENCE | length | MCF10A | MCF7 | MDA | MIRBASE | STAR | LOCATION |
| hsa-miR-B1 | GGCTGGTCCGAAGGTAGTGAGTTATCT | 27 | 585 | 1347 | 1251 | Novel | No | chr11:13304324–13304433[−] |
| hsa-miR-B2 | CCTGCAGTAGCTGTTTCT | 18 | 1 | 0 | 1651 | Novel | No | chr9:73809165–73809274[−] |
| hsa-miR-B3 | GGCTGGTCCGAGTGCAGTGGTGTTTACAACT | 31 | 257 | 433 | 1007 | Novel | No | chr13:98986564–98986673[−] |
| hsa-miR-B4 | TAAAAGTAATTGTGGTATTTGC | 22 | 0 | 0 | 57 | 1 mismatches with hsa-548d-5p | No | chr1: 81947441–81947550[+] |
| hsa-miR-B5 | GAGCCCGGAGGGCGAGG | 17 | 639 | 8 | 396 | Novel | No | chr1:1172913–1173022[−] |
| hsa-miR-B6 | AAGGTAGATAGAACAGGTCTTG | 22 | 364 | 528 | 215 | perfect match with mouse, dog, cow | Yes(22) | chr15:81221792–81221898[+] |
| hsa-miR-B7 | CTGAGCAACATAGCGAGACCCCGTCTCTA | 29 | 468 | 214 | 340 | close to hsa-miR-1303 (chr5) in diff loc | No | chr16:3297692–3297801[−] |
| hsa-miR-B8 | AAGCCATGTTACGAGCCTTAAGG | 23 | 20 | 28 | 15 | Novel | Yes(14) | chr6:133180097–133180198[+] |
| hsa-miR-B9 | TGTGGTCTAGTGGTTAGGAT | 20 | 169 | 111 | 157 | close to cow (bta-mir-2476) | No | chrY:586300–586319[+] |
| hsa-miR-B10 | GGGGGTGTAGCTCAGTGGTAGAGCA | 25 | 209 | 46 | 92 | 1 mismatch with mmu-miR-1959 | No | chr6:28834104–28834213[−] |
| hsa-miR-B11 | GGCCAGCCACCAGGAGGGCTGC | 22 | 1 | 15 | 14 | Novel | Yes(5) | chr20:49502834–49502934[−] |
| hsa-miR-B12 | TGTTGGTGTTTATGTTG | 17 | 0 | 0 | 78 | Novel | No | chr8:107080026–107080135[+] |
| hsa-miR-B13 | CCGTGTTTCCCCCACGCTTT | 20 | 49 | 29 | 206 | Novel | No | chr17:8031210–8031319[+] |
| hsa-miR-B14 | GTCTCCTTGTTATGGGGCAGTGCAG | 25 | 153 | 10 | 29 | Novel | No | chr1:153915582–153915691[−] |
| hsa-miR-B15 | AAAGACATAGTTGCAAGATGGG | 22 | 0 | 0 | 80 | Novel | No | chr20:43767137–43767246[−] |
| hsa-miR-B16 | GTGGGTGATGTTTGCTGACA | 20 | 32 | 2 | 28 | Novel | No | chr22:40334774–40334883[−] |
| hsa-miR-B17 | GGGCTGTGATGTTTATTAGCTTCTGAGCTC | 30 | 180 | 16 | 36 | Novel | No | chr17:38359009–38359118[−] |
| hsa-miR-B18 | GATGGTGATGATGCTGGTC | 19 | 0 | 0 | 26 | Novel | chr7: 91108780–91108889[+] | |
| hsa-miR-B19 | AAAAGGGGGCTGAGGTGGAG | 20 | 8 | 0 | 133 | Novel | No | chr11:121527997–121528099[−] |
| hsa-miR-B20 | CCAAGGAAGGCAGCAGG | 17 | 19 | 22 | 125 | Novel | No | chr1:202811178–202811287[−] |
| hsa-miR-B21 | TATGTGTGTGTGCTTGTATAT | 21 | 1 | 0 | 88 | close to mouse (mmu-miR-669) | No | chr8:129122276–129122385[+] |
| hsa-miR-B22 | TCCCCAGCACCTCCACCA | 18 | 114 | 1 | 11 | Novel | No | chr7:73108306–73108415[−] |
| hsa-miR-B23 | TGAGGAATATGGTGATC | 17 | 0 | 0 | 48 | Novel | No | chr1:33878065–33878174[+] |
| hsa-miR-B24 | GTTCTTGTAGTTGAAATACAACGATG | 26 | 29 | 12 | 51 | Novel | No | chr5:105917028–105917137[+] |
| hsa-miR-B25 | TTGGCCATGGGGCTGCGCGG | 20 | 33 | 42 | 36 | Novel | No | chr19:764562–764671[+] |
| hsa-miR-B26 | ATCCCACCACTGCCACCA | 18 | 59 | 21 | 3 | one mismatch with hsa-miR-1260 | No | chr11:95714237–95714346[+] |
| hsa-miR-B27 | CCAGGAATCCTGCTGTGGTGGA | 22 | 8 | 0 | 50 | Novel | Yes(3) | chr11:121532093–121532199[−] |
| hsa-miR-B28 | TGTCCTTGCTGTTTGGAGATAA | 22 | 68 | 21 | 8 | close to cow (bta-mir-2355) | Yes(14) | chr2:207682944–207683054[−] |
| hsa-miR-B29 | ATGTGGGCTAGTTTCAGACAGGT | 23 | 7 | 11 | 42 | Novel | No | chr1:28778841–28778945[−] |
| hsa-miR-B30 | CACCTTGCGCTACTCAGGTCTGC | 23 | 13 | 47 | 15 | Novel | No | chr22:29457597–29457619[+] |
Candidate miRNAs in bold were used to evaluate miRNA expression using Northern blotting.