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. 2011 Feb 8;6(2):e16869. doi: 10.1371/journal.pone.0016869

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Crellin et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Southern blot of genomic DNA digested with HindIII/NotI/XbaI. Lane 1, DNA molecular weight DNA markers of the sizes indicated (kilobases, kb); lane 2, wild-type M. smegmatis mc²155; lane 3, single crossover strain used to derive the conditional knockout; lane 4, conditional knockout of MSMEG_6382, designated 6382CKO.