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. 2011 Feb 8;6(2):e16679. doi: 10.1371/journal.pone.0016679

Figure 3. Age-associated methylation changes in fibroblasts and MSC.

Figure 3

Mean DNA methylation of 27,578 CpG sites was compared between fibroblasts from young donors (<23 years, eight samples) and from elderly donors (>60 years; six samples) by scatter plot analysis. 75 CpG sites revealed more than 15% age-associated differential methylation (indicated as black spots; A). Scatterplot analysis of MSC from young (<50 years, four samples) and elderly donors (>50 years, four samples) demonstrated that 1060 CpG have more than 15% age-associated DNA methylation changes (B). Comparison of fibroblasts revealed that 30 CpG sites were overlapping differentially methylated upon aging in MSC and fibroblasts (black spots; C). This is highly significantly more than expected by chance alone but differential methylation often occurred in opposite directions.