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. 2011 Feb 8;5(2):e955. doi: 10.1371/journal.pntd.0000955

Figure 2. Formalin preservation allows specific discrimination of infected rat fecal supernatant from uninfected fecal supernatant.

Figure 2

(A) Effect of assay specificity after various treatments. Pooled uninfected fecal supernatant collected from 6 rats (nRFS) and infected rat fecal supernatant collected from 2 rats infected with S. ratti (13 and 21 dpi) (iRFS) were extracted simultaneously in PBS-T and 4% formalin (4%F). Only extraction in formalin led to a positive discrimination between nRFS and iRFS. All points are mean ± SEM of OD values obtained from duplicate samples. (B) Detection of known amounts of E/S products diluted in PBS or uninfected rat fecal supernatant (nRFS) extracted in 4% formalin. The lowest concentration of E/S products detected was 80 ng/ml and 325 ng/ml when diluted in PBS and nRFS, respectively. All points are mean ± SEM of OD values obtained from 4 samples.