Figure 3. Chemical structure of free and immobilized 4-[4-Amino-5-(2-methoxy-benzoyl)-pyrimidin-2-ylamino]-piperidine-1-carboxylic acid (4-hydroxy-phenyl)-amide (SCYX-7434).

SCYX-7434 was coupled to activated sepharose 6B via the carbodiimide reaction, as described in materials and methods. Control capped sepharose 6B was produced by the addition of ethanolamine to block the reactive groups.