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Distribution of PI4KIIα and PI4P synthesis in the subcellular fractions. Endomembrane fractions were prepared. and equal-volume samples from each gradient fraction were separated by SDS-PAGE and probed by Western blotting against (A) PI4KIIα (1:5 hybridoma culture supernatant) and (B) distribution of PI4P synthesis in the gradient fractions. PI4P, phosphatidylinositol 4-phosphate; PI4KII, type II phosphatidylinositol 4-kinase.
