Table 1.

Protein identified from the proteomes of tumors in naïve and vaccinated Balb/c mice

Spot	Protein	Gene	Swiss-Prot Accession No.	Observed m/z and predicted peptide sequencea)	Sequence coverage (%)
1	α-Actin	ACTC1	A54728	976.44 (19–28); 1130.53 (197–206); 1198.69 (29–39); 1500.68 (360–372); 1515.71 (85–95); 1790.83 (239–254); 1955.97 (96–113)	23
2	α-Actin	ACTC1	A54728	976.44 (19–28); 1130.53 (197–206); 1198.69 (29–39); 1500.68 (360–372); 1515.71 (85–95); 1790.83 (239–254); 1955.97 (96–113)	23
3	Adenylate kinase 1	AK1	Q9R0Y5	994.40 (117–124); 1130.61 (26–37); 1495.73 (48–60); 1539.73 (100–113); 2209.09 (166–183)	30
4	β-enolase	ENO3	P21550	1166.54 (184–193); 1380.62 (16–28); 1475.66 (413–426); 1804.83 (33–50)	12
5	β-enolase	ENO3	P21550	1166.54 (184–193); 1380.62 (16–28); 1475.66 (413–426); 1638.85 (90–103); 1804.83 (33–50)	15
6	Creatine kinase M chain	CKM	A23590	1002.47 (97–105); 1062.56 (33–41); 1231.63 (87–96); 1269.74 (305–314); 1507.70 (117–130); 1643.81 (224–236); 1723.74 (210–223)	20
7	Creatine kinase fragment 1	CKM	A23590	1002.47 (97–105); 1062.56 (33–41); 1083.53 (108–116); 1130.63 (87–96); 1231.63 (87–96); 1507.70 (117–130); 1723.74 (210–223)	17
8	Creatine kinase fragment 2	CKM	A23590	1002.47 (97–105); 1062.56 (33–41)b; 1083.53 (108–116); 1231.63 (87–96); 1507.70 (117–130); 1723.74 (210–223)	17
9	Hemoglobin beta	HBB-B1	P02088	934.50 (9–17); 1136.68 (133–144); 1274.76 (31–40); 1294.66 (121–132); 1302.65 (18–30); 1756.88 (67–820)	38
10	Hemoglobin beta dimer	HBB-B1	P02088	1126.57 (96–104); 1274.76 (31–40)b); 1436.81 (133–146); 1756.88 (67–820)	33
11	Hemoglobin beta dimer	HBB-B1	P02088	1126.57 (96–104); 1274.76 (31–40); 1436.81 (133–146); 1756.88 (67–820)	33
12	Prohibitin	PHB	P24142	1058.52 (187–195); 1062.50 (149–157); 1149.61 (134–143); 1185.67 (84–93); 1460.62 (106–117)	18

^a)The predicted amino acid sequence for signature m/z fragments is given in parentheses.

^b)Oberved m/z (predicted peptide sequence) was confirmed by MS/MS sequencing.