Table 2.

Quantitative analysis of the changes in the proteomes of tumors in naïve and vaccinated Balb/c mice

Spot	Protein	Protein levels				% Naive subjects
		Naive		Vaccinated
		CB	SN	CB	SN	CB	SN
1	α-Actin	2.12 ± 0.21	0.91 ± 0.57	0.10 ± 0.01b)	0.99 ± 0.63	4.7	108.8
2	α-Actin	2.05 ± 0.13	1.73 ± 1.43	0.09 ± 0.02b)	1.98 ± 1.02	4.4	114.5
3	Adenylate kinase 1	0.10 ± 0.01	0.19 ± 0.03	0.35 ± 0.04b)	0.70 ± 0.05b)	350.0	368.4
4	β-enolase	0.19 ± 0.05	0.26 ± 0.05	0.33 ± 0.08a)	0.32 ± 0.07a)	173.7	123.1
5	β-enolase	0.20 ± 0.04	0.17 ± 0.04	0.42 ± 0.16a)	0.34 ± 0.03a)	210.0	200.0
6	Creatine kinase M chain	0.20 ± 0.08	0.31 ± 0.10	2.13 ± 0.27b)	1.57 ± 0.07a)	1065.0	506.4
7	Creatine kinase fragment 1	0.13 ± 0.02	0.12 ± 0.01	0.44 ± 0.18a)	0.45 ± 0.10a)	338.5	375.0
8	Creatine kinase fragment 2	0.17 ± 0.03	0.91 ± 0.02	0.09 ± 0.01b)	0.49 ± 0.02b)	52.9	53.8
9	Hemoglobin beta	0.11 ± 0.05	0.25 ± 0.09	0.41 ± 0.15a)	0.52 ± 0.11a)	390.9	208.0
10	Hemoglobin beta dimer	0.10 ± 0.02	0.23 ± 0.04	0.33 ± 0.03b)	0.72 ± 0.17a)	330.0	313.0
11	Hemoglobin beta dimer	NI	1.21 ± 0.14	NI	3.03 ± 0.17a)	NI	250.4
12	Prohibitin	NI	1.01 ± 0.07	NI	2.34 ± 0.21a)	NI	231.7
13	NI	NI	0.26 ± 0.08	NI	2.21 ± 0.18b)	NI	850.0
14	NI	NI	0.26 ± 0.09	NI	2.17 ± 0.11b)	NI	834.6
15	NI	NI	2.52 ± 0.22	NI	2.13 ± 0.12a)	NI	84.5

The protein level of each spot is expressed as a percentage of total spot volume in the 2-DE gel. Protein expression levels were quantified and compared using PDQuest software (Bio-Rad). Differences in protein level are expressed as a percent of the naive subjects. Results represent mean ± SD values. Statistical analysis of data from three independent experiments was performed using Student's t-test. Differences from the group of naïve subjects reaching statistical significance are indicated by a) (p < 0.05) or b) (p < 0.01). NI, protein not identified; CB, protein spots selected from CBB R-250 stained 2-DE gels; SN, protein spots selected from silver nitrate stained 2-DE gels