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. Author manuscript; available in PMC: 2012 Apr 4.
Published in final edited form as: Anal Chem. 2010 Oct 4;82(21):10.1021/ac101911a. doi: 10.1021/ac101911a

Figure 1.

Figure 1

SPR sensorgrams from the preparation of AECPBA (left) and hydroxyl-terminated control (right) surfaces. In each case, the carboxymethyl dextran surfaces were first treated with pH 7.40 HBS-EP (0.010 M HEPES, 0.15 M NaCl, 3.0 × 10−3 M EDTA, 0.0050% v/v Tween-20), then 70 μL of 0.070 M NHS/0.20 M EDC was injected, followed by a minimum of 4 injections of 70 μL of 0.025 M AECPBA in pH 8.50 borate buffer for the AECPBA surface or 70 μL of pH 8.50 borate buffer followed by 40 μL of 1.0 M ethanolamine (pH 8.50) for the hydroxyl-terminated control surface. Capping of any remaining NHS sites on the AECPBA surface was achieved by injecting 40 μL of pH 8.50, 1.0 M ethanolamine.

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