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. Author manuscript; available in PMC: 2012 Apr 4.
Published in final edited form as: Anal Chem. 2010 Oct 4;82(21):10.1021/ac101911a. doi: 10.1021/ac101911a

Figure 2.

Figure 2

Representative SPR sensorgram for avidin binding on and elution from the AECPBA surface. The binding experiment was performed with 20 μL of 5.16 × 10−6 M avidin in pH 9.00 glycine-buffered saline (0.050 M glycine, 0.15 M NaCl) at a flowrate of 2 μL min−1, while the elution (regeneration) experiment was performed with 65 μL of pH 10.00 borate-buffered saline (0.10 M borate, 0.30 M NaCl) at a flowrate of 10 μL min−1. The flowrate during the buffer run was kept at 2 μL min−1. The AECPBA surface was prepared through direct EDC coupling.

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