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. Author manuscript; available in PMC: 2012 Apr 30.
Published in final edited form as: N Biotechnol. 2010 Jul 15;28(3):239–249. doi: 10.1016/j.nbt.2010.07.003

Figure 2.

Figure 2

Comparison of translation and purification results associated with different transcription buffers, wheat germ extracts, and translation buffers. Transcription was performed in either LM or TM buffer. The small-scale translation of DCN1 was performed in dialysis cups with WEPRO8240H (8) or WEPRO2240H (2) and either SUB-AMIX SG (S) or DB (D) translation buffer. A. SDS-PAGE of reaction mixtures performed in different conditions. B. Samples from A after small-scale batch IMAC purification. The triangle marks the migration position of His6-DCN1.