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Detergent retention in 10,000 MWCO ultrafiltration concentrators. Buffers containing the indicated detergents were centrifuged in the same concentrators used by the Protemist10/100 robots. After centrifugation at 3000×g for 10 min, thin layer chromatography was performed on the original sample (O), retentate (R), and flow-through (F) and visualized by iodine vapor staining. Black asterisk, non-detergent buffer components; red asterisk, FC-12; green asterisk, CHAPS.
