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. Author manuscript; available in PMC: 2012 Apr 30.
Published in final edited form as: N Biotechnol. 2010 Jul 15;28(3):239–249. doi: 10.1016/j.nbt.2010.07.003

Figure 6.

Figure 6

Detergent-mediated expression of the bR in the Protemist XE. A. Results of small-scale translation in dialysis cups with the indicated detergent additives. B. CHAPS partitioning in the Protemist XE with VIVAFLOW 50 ultrafiltration membrane. 0.2% CHAPS was added to both the reaction and feed buffers in a mock translation buffer with no extract in the reaction buffer. Samples were removed before start (0) and after 7 and 18 cycles of operation and analyzed by thin layer chromatography and iodine vapor staining. Standards of known concentrations of CHAPS are shown. C. Time course of bR synthesis with 0.2% CHAPS and 0.1 mM all-trans-retinal included in reaction and feed buffers. Synthesized bR was determined by tryptophan fluorescence (24) using Stain-Free SDS-PAGE (BioRad).