A, examples of ICa-L. B, averaged I–V relationships of ICa-L in VMs from control (Ctr), LE and HE mice. The maximal ICa-L in Ctr, LE and HE myocytes were −12.77±0.84, −23.30±2.16, and −33.09±4.01pA/pF, respectively. C, Example of Ca2+ transients recorded with indo-1 in VMs from Ctr, LE and HE mice (0.5Hz). D, There was no difference in diastolic Ca2+ in control (0.63±0.02), LE (0.65±0.03) and HE (0.62±0.03) VMs but increased amplitudes of systolic Ca2+ in DTG VMs (ctr: 0.72±0.02; LE: 0.81±0.05; HE: 0.96±0.04). E, fractional shortening was enhanced β2a (Ctr: 5.0±0.6%; LE: 8.2±1.1%; HE: 9.6±0.9%). F, Western blots of total PLB, pSer16 PLB, pThr17 PLB, NCX, SERCA, α1c, RyR and GAPDH (internal control) in control, LE and HE heaerts. G, Averaged normalized protein expression levels. In B: @: p<0.01 vs. Ctr; #: p<0.05 vs. LE; $: p<0.05 vs. Ctr; &: p<0.01 vs. Ctr. In D & E: *: p<0.05; **: p<0.01. “n” is the number of cells from at least 3 animals.