A & B, The effects of different MOIs (0, 5, 10, 50 and 100) of Adβ2a or AdGFP on myocyte volume. β2a expression significantly increased the volume of AFVMs after 4 days of culture. C, Normalized protein/DNA ratio in cells infected with Adβ2a or AdGFP at the MOI of 5. D, The effects of Adβ2a (MOI 5) or AdGFP (MOI 5) on the surface area of NRVMs. β2a overexpression increased NRVM surface area and sarcomere organization. *: p<0.05 vs. AdGFP at the same MOI; **: p<0.01 vs. AdGFP at the MOI of 5.