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Bodipy-C16 binding to kinases. 10 nM of Bodipy-C16 was added to PBS buffer in the absence of kinase and in the presence of 40 nM and 80 nM of kinases, PKR-pWT, Akt1, MAPKAPK3, and CDK4. Lysozyme was used as a negative control under the same condition. After 5 min of incubation, the fluorescence polarization was measured at 488/520 nm using a spectrofluorometer. Representative data point shown is for the average of three measurements and the error bars indicate the standard deviations.
