Figure 4.

Peptide 234 effects on basal and kisspeptin-10-stimulated plasma LH in intact and castrated male rats. A, Peptide 234 inhibits kisspeptin-10-induced LH secretion in intact male rats. The animals were infused with 1 nmol peptide 234 at 0 and 60 min, followed by infusion of 100 pmol kisspeptin-10 with 1 nmol peptide 234 at 120 min. The peptide significantly inhibited LH production over the following 2 h (n = 10; *p < 0.05). B, Castrated male rats were given three infusions of 1 nmol peptide 234 at 0, 60, and 120 min, which significantly inhibited LH secretion after 240 min with 1 nmol peptide 234 (n = 10; p < 0.05). Bars show mean ± SEM.