Table 2. Differentially expressed proteins in the cerebellum of PS1KI (CTRL) and 3 × Tg-AD mice.
| Spot no. | Protein (symbol) | Accession numbera | Probability- based Mowse score (score)b | Sequence coverage (%) | nmatched masses/ nsubmitted masses/ nmatched peptides containing a missed cleavage site | HRc/ ELDPd/ MCe | PMFscoref | Theoretical MW (Da)/pI | Experimental MW (kDa)/pI | Mean density ratio (s.d.)g | Peptide sequence (start-end, Mascot scoreb, ion scoreh) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 3 | Creatine kinase, brain (CKB) | 10946574 | 78 | 28 | 9/21/1 | 0.42/7/12.0 | 85 | 42971/5.40 | 51.8/5.4 | 2.5 (0.2) | |
| 5 | Valosin-containing protein, isoform CRA_a (VCP) | 148670553 | 172 | 28 | 17/24/4 | 0.71/9/25.4 | 120 | 90868/5.14 | 119.8/4.9 | 2.5 (0.3) | |
| 6 | Glutamate deydrogenase 1 (GLUD1) | 148692928 | 187 | 34 | 16/30/3 | 0.53/10/18.5 | 172 | 54527/7.66 | 66.0/7.9 | 3.2 (0.3) | |
| 7 | Hypothetical protein L0C433182 (ENO1) | 70794816 | 70 | 18 | 9/23/0 | 0.39/9/8.5 | 101 | 47453/6.37 | 55.9/6.7 | 2.0 (0.3) | |
| 8 | Lactate dehydrogenase B (LDHB) | 6678674 | 173 | 49 | 16/30/4 | 0.53/8/18.0 | 102 | 36834/5.70 | 39.3/5.8 | 3.1 (0.4) | |
| 11 | Isocitrate dehydrogenase 3 (NAD+) α, isoform CRA_a (IDH3A) | 148693871 | 143 | 39 | 14/21/1 | 0.67/12/13.7 | 138 | 35021/5.86 | 42.7/5.5 | 1.9 (0.3) | |
| 12 | mKIAA0098 protein (CCT5) | 37359776 | 140 | 28 | 13/20/2 | 0.65/9/16.8 | 111 | 60143/5.72 | 78.1/6.0 | 2.7 (0.2) | |
| 13 | Dihydropyrimidinase-like 2 (DPYL2) | 40254595 | 167 | 38 | 14/20/0 | 0.70/14/23.8 | 169 | 62638/5.95 | 83.2/6.4 | 7.5 (0.9) | |
| 16 | Creatine kinase, brain (CKB) | 10946574 | 156 | 49 | 14/24/1 | 0.58/12/21.1 | 145 | 42971/5.40 | 51.6/5.3 | 3.5 (0.4) | |
| 18 | Carbonic anhydrase 2 (CA2) | 157951596 | 118 | 47 | 7/12/0 | 0.58/7/13.7 | 88 | 29129/6.49 | 30.8/7.6 | 3.6 (0.3) | |
| 19 | Phosphoglycerate mutase 1 (PGAM1) | 114326546 | 166 | 47 | 14/21/6 | 0.66/2/13.6 | 38 | 28928/6.67 | 29.9/7.3 | 1.6 (0.2) | R.VLIAAHGNSLR.G (124–134, 74, 54) R.DAGYEFDICFTSVQK.R (47–61, 74, 55) K.NLKPIKPMQFLGDEETVR.K (166–183, 65, 44) |
| 22 | Rho GDP-dissociation inhibitor 1 (ARHGDA) | 21759130 | 98 | 54 | 10/32/5 | 0.31/0/12.7 | 15 | 23450/5.12 | 28.4/4.8 | 2.2 (0.2) | R.VAVSADPNVPNVIVTR.L (59–74, 64, 40) R.FTDDDKTDHLSWEWNL TIK.K (181–199, 56, 34) |
| 23 | Ubiquitin C-terminal hydrolase L1 (UCHL1) | 188219614 | 82 | 30 | 5/17/1 | 0.29/3/7.5 | 40 | 25164/5.33 | 28.5/4.9 | 1.9 (0.3) | K.NEAIQAAHDSVAQEGQCR.V (135–152, 106, 86) K.QTIGNSCGTIGLIHAVAN NQDK.L (83–104, 75, 54) |
| 24 | Glyoxalase 1 (GLO1) | 165932331 | 123 | 45 | 8/16/1 | 0.50/6/9.4 | 73 | 20967/5.24 | 26.7/4.8 | 2.7 (0.3) | K.DFLLQQTMLR.I (29–38, 66, 46) R.GFGHIGIAVPDVYSACK.R (124–140, 97, 76) K.FSLYFLAYEDKNDIPK.D (68–83, 67, 46) |
PMFscore values lower than 79 are in bold.
a
gi number in NCBInr database.
b
Mascot score is given as S= −10 × log (P), where P is the probability that the observed match is a random event.
c
Hit ratio (HR)=nmasses matched/nmasses submitted for database search.
d
Excess of limit-digested peptides (ELDP)=(nmatched peptide having no missed cleavage)−(nmatched peptides containing a missed cleavage site).
e
Mass coverage (MC)=(% sequence coverage/100) × MW(kDa)
f
Peptide mass fingerprint score (PMFscore)=(HR × 100)+MC+(ELPD × 10).
g
Mean (s.d.), n=4.
h
Ion score is given as S= −10 × log (P), where P is the probability of the peptide being a random match to its corresponding MS/MS spectrum