Table 1. Kinetic parameters of trimer–monomer complex using surface plasmon resonance.

	DR4-Ig
Protein	ka (/M/s)a	kd (s−1)b	KD (nM)c
WT	(7.45±0.4) × 105	(1.66±0.3) × 10−4	0.22±0.06
4C7	(28.1±1.7) × 105	(0.59±0.3) × 10−4	0.021±0.01
4C9	(23.5±1.4) × 105	(0.58±0.4) × 10−4	0.025±0.02
	DR5-Ig
WT	(13.9±1.5) × 105	(0.65±0.3) × 10−4	0.033±0.03
4C7	(1.14±0.5) × 105	(8.48±0.8) × 10−4	7.41±4.2d
4C9	(19.0±0.7) × 105	(23.1±1.2) × 10−4	1.22±0.2d

^aAssociation rate constant.

^bDissociation rate constant.

^cEquilibrium dissociation constant (k_d/k_a). Each value was calculated using the BIAcore 3000 and the BIA evaluation 4.1 software.

^dCurves showed heterogeneity, a phenomenon that may be correlated to partial glycosylation of receptors