Fig. 1.

S. pombe hst4Δ cells are more sensitive to H₂O₂. (a) Wild-type (WT) and hst4Δ S. pombe cells in exponential growth were treated with 0, 1, 2, and 3 mM of H₂O₂ for 30 min, diluted for every 4-fold, and 4 μl are spotted onto YES media. (b) Wild-type (WT) cells and three HDAC mutants clr6Δ, hst4Δ, and sir2Δ were treated with or without 2 mM of H₂O₂ for 30 min, diluted for every 5-fold, and 4 μl are spotted onto YES media.