Fig. 2.

SpHst4 protein level decreases following H₂O₂ treatment in wild-type (WT) but not in myh1Δ cells. S. pombe Hu1500 (a) and Hst4-MYC myh1Δ (b) were treated with 5 mM of H₂O₂ for 30 min and then recovered for 0 (T0), 1 (T1), or 3 (T3) hours or remained untreated (UN). Total cell extracts were prepared, separated on 4-20% gradient SDS-polyacrylamide gels and subjected for Western blotting with a mixture of antibodies against c-Myc and histone H3. The amounts of SpHst4-Myc were quantitated relative to those of H3 (Relative AMT).