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. Author manuscript; available in PMC: 2012 Jan 21.
Published in final edited form as: J Mol Biol. 2010 Nov 24;405(3):653–665. doi: 10.1016/j.jmb.2010.11.037

Fig. 6.

Fig. 6

The association of SpHus1 and SpHst4 to telomeres is dependent on SpMyh1 and the association of SpMyh1 to telomeres is enhanced in hst4Δ cells. (a) ChIP was performed similarly to Fig. 5 except that both Hu1500 (WT) and Hst4-MYC myh1Δ (Δ) cells were used. Cells were treated with 5 mM H2O2 for 30 min and then recovered for one hour (T1) or left untreated (UN). c-Myc antibody (columns 1-4) and SpMyh1 antibody (columns 5-8) were used to precipitate SpHst4 and SpMyh1 associated DNA, respectively. Fold enrichment at Telo was calculated relative to ade6+. Statistical significance between wild-type and myh1Δ is indicated with a star. (b) ChIP was performed similarly to (a) except that Hus1-MYC (WT1), Hus1-MYC myh1Δ (myh1Δ), Hu303 (WT2) and Hu1481 (hst4Δ) cells were used. c-Myc antibody (columns 1-2) and SpMyh1 antibody (columns 3-6) were used to precipitate SpHus1 and SpMyh1 associated DNA, respectively. Fold enrichment at Telo was calculated relative to ade6+. Statistical significance (P < 0.02) between wild-type and hst4Δ is indicated with a star.