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. Author manuscript; available in PMC: 2012 Feb 4.
Published in final edited form as: Cell Stem Cell. 2011 Feb 4;8(2):188–199. doi: 10.1016/j.stem.2010.12.006

Figure 5. Regulation of ISC proliferation by modulating the intracellular redox state.

Figure 5

A: Increased ISC proliferation in response to loss of mitochondrial electron transport chain components (NADH:ubiquinone reductase 75kD and 42kD subunit precursors; ND75RNAi and ND42RNAi). PH3+ cells per gut are quantified (Average and SEM, Student's T-test, N in bars).

B, C: Representative images and quantification of DHE fluorescence in intestines over-expressing Gclc or Jafrac1 in ISCs (7 day old flies, Average and SEM, Student's T-test, N in bars; esgGFP, green; DHE, red).

D: Cell numbers in MARCM clones over-expressing Gclc or Jafrac1 quantified at 4 and 14 days, or at 7 days after heat shock (average cell numbers and standard deviations; Student's T-test).

E: Quantification of mitotic figures in intestines of 10 day-old flies over-expressing Gclc or Jafrac1 under the control of esgG4::UASGFP (Average and SEM, Student's T-test).

F: Cnc-mediated repression of ISC proliferation in response to Paraquat exposure requires jafrac1 gene function. CncC was over-expressed under the control of esgGal4 in wild-type or jafrac1KG05372 heterozygous backgrounds (Average and SEM, Student's T-test).

See also Figure S5.