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. Author manuscript; available in PMC: 2011 Nov 12.
Published in final edited form as: Cell. 2010 Nov 12;143(4):592–605. doi: 10.1016/j.cell.2010.10.032

Figure 2. Phenotype of LZ and DZ cells.

Figure 2

(A) Diagrammatic representation of the experimental protocol.

(B) Examples of LZ (top row) and DZ (bottom row) multiphoton photoactivation. Collapsed Z-stacks (40 μm, 5 μm steps). Scale bar = 50 μm. Imaged at λ=940 nm before and after photoactivation at λ=830 nm.

(C–E) Chemokine receptor and BCR expression, DNA content (DAPI), and forward and side scatter in photoactivated LZ or DZ cells. GC cells gated as live/single, CD45.1+, FAShi. Non-GC cells (shown for comparison) gated as Igλ+FAS− Left panels: quantification of flow cytometry data. Each symbol represents one experiment (pools of 3–5 mice per experiment).