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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Cell Signal. 2010 Dec 3;23(4):660–665. doi: 10.1016/j.cellsig.2010.11.017

Figure 4. TAK1 lysine 158 but not lysine 209 is required for IL-1β- and TNFα-induced IKK, JNK and p38 activation.

Figure 4

Expression of the TAK1-K158R mutant, but not -K209R mutant, inhibits IL-1β- and TNFα-induced p38, JNK, IKK phosphorylation. TAK1-deficient MEF cells were transduced with the retrovirus encoding TAK1-wild-type, -K158R or -K209R mutant and subsequently selected with puromycin (3 μg/ml) to establish the TAK1-deficient MEF cell lines with the stable expression of either TAK1 wild-type, -K158R or –K209R mutant. The reconstituted TAK1-deficient MEF cells were untreated or treated with IL-1β (10 ng/ml) (A) or TNFα (10 ng/ml) (B) for the time points indicated and subsequently harvested. Whole cell extracts were subjected to SDS-PAGE and immunoblotted with antibodies indicated.