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. Author manuscript; available in PMC: 2011 Feb 9.
Published in final edited form as: Environ Sci Technol. 2008 Jul 1;42(13):5014–5019. doi: 10.1021/es8001508

FIGURE 1.

FIGURE 1

Plasmid DNA relaxation induced by Fenton-like reaction of cerium with hydrogen peroxide. The migration of supercoiled (form 1) and nicked supercoiled (form 2) plasmid (pBR322) was analyzed upon incubation of cerium with hydrogen peroxide (88 mM) in Tris buffer as described in methods. Aliquots were taken every 10 minutes for 1 h, and the reaction was stopped using 10 mM EDTA. DNA was separated by gel electrophoresis (0.7% agarose gel). C represents control DNA not treated and (–) indicates cerium chloride in the absence of peroxide incubated for 60 min. (A) Cerium chloride (100 μ) or (B) Cerium chloride (20 μM) with tert-butyl hydroperoxide (88 mM). A representative experiment is shown from at least three independent experiments with reproducible results.

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