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. 2009 Sep 1;32(3):652–665. doi: 10.1590/S1415-47572009000300033

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Copyright © 2009, Sociedade Brasileira de Genética.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Two dimensional gel electrophoresis of Leishmania amazonensis flagellar fraction. Proteomic analysis after visualization by silver staining. Imaging covering narrow range IPG strips of pH 3-10 - observed in the gel top. The standard of molecular mass range used is 14400 Da to 97000 Da; -1 is a software configuration parameter. Six (06) putative spots for coronin are circled and indicated by a red arrow; four (04) Arp2/3 putative spots are circled and pointed by a yellow arrow. The molecular weight (in KDa) and the corresponding isoeletric point of each protein are shown in white.