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. 2009 Sep 1;32(3):466–469. doi: 10.1590/S1415-47572009000300007

Table 1.

Oligonucleotide primers for PCR amplification of the exons of PVR and PVRL2.

Amplicon Primer sequences Amplicon size (bp)
PVR
Exon 1 5' AGAGCGACGGGCGCCGGGAA 3'
5' ACTGCGCGGGGGTCACTCAC 3'
165
Exon 2 5' TTCTCTTCGGTTCTCCGCAG 3'
5' CCCCAAAACCCCCTGCTC 3'
388
Exon 3 5' GCTTTTGTTCCTCTTCCCAG 3'
5' GCTGACTTGGGCACACTCAC 3'
337
Exon 4 5' TCTGTATCCATTTCCTGCAG 3'
5' CCCTGAGACCCAGGACTCAC 3'
158
Exon 5 5' CACCTTTCTGTCTCTCTCCCAG 3'
5' CCACCCAGGGAGTTCCTCAC 3'
189
Exon 6 5' CCTGTTTCCTTCTCTTTCAG 3'
5' GTAGGTGCTCAATTACGGCA 3'
216
Exon 7 5' TTCCCCTCCTATTTCCCCAG 3'
5' AGCTCCAACACTGCACTTAC 3'
72
Exon 8 5' ATTTGAAAACCCTCTTCTAG 3'
5' GGTCCAACTCTGGAGGCCCA 3'
158

PVRL2
Exon 1 5' CTACTAAACCGCCCAGCCGA 3'
5' CGGTTTCCAGGAGCAGCAGC 3'
169
Exon 2 5' GTGGCCCTGCCTGGAGGTGT 3'
5' TGACCCGCAAGGGGATGCTC 3'
530
Exon 3 5' CTCCTCTGCTGAGTGTTTGT 3'
5' GTAGACAGTGCTTTAGAGAA 3'
437
Exon 4 5' CTATCTGCTAACTTGTCCAC 3'
5' TTAGATCCAGGAGTCCAGGC 3'
258
Exon 5 5' TCTTTAGGGATGAGGCCTGTG 3'
5' AAGTCCTGAAGGGCAGAACT 3'
290
Exon 6 5' CCCAGAGCGATCCTCGTGAT 3'
5' AACCAGTCTGGAACCCTAGG 3'
550
Exon 7 5' GATGGTCGCTTGGAATAAGG 3'
5' CTCACCCTACCCCATACTC 3'
295
Exon 8 5' GTGCCATAACCCCGGAGTCA 3'
5' GCCAGGCCCCTCCCAGCCCT 3'
205
Exon 9 5' GGCCTGGCAGGGAGAAGCTG 3'
5' TTGCCAGGCTTGACCCCTGG 3'
228
Exon 10 5' AAGAGCAGATTGGTAATCTG 3'
5' GGCACTAGATCCTTGGCAAG 3'
411