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. 2010 Mar 1;33(1):141–145. doi: 10.1590/S1415-47572010000100024

Figure 1.

Figure 1

A. Intron prediction for the Alpinia calcarata PKS sequence carried out at the NetPlantGene web server. The arrows represent putative splice sites. B. A donor splice site predicted at 217 bp and an acceptor splice at the 310 bp position. C. Amplification of a core fragment of the Alpinia calcarata PKS gene. Lanes (1) PCR product obtained from genomic DNA of Zingiber officinale, used as positive control, (2) PCR product obtained from genomic DNA of Alpinia calcarata, (3) negative control, and (4) 100 bp DNA ladder.