Table 1.
Strain or plasmid | Characteristics | Source or reference |
Escherichia coli | ||
DH5α | F- φ80dlacZΔM15 Δ (lacZYA-argF)U169 endA1 deoR recA1 hsdR17(rK- mK+) phoA supE44 λ-thi-1 gyrA96 relA1 | Gibco |
Xanthomonas axonopodis pv. citri | ||
306 | Wild type, Apr | R.P. Leite |
30E9 | Kanr, XpsD- | This study |
ΔxpsD | Kanr, site-directed XpsD- | This study |
Plasmid | ||
pCR2.1-TOPO | vector pUC18 derivative, kanr, bla (Apr), lacZ | Invitrogen |
TOPO-xpsD | PCR-amplified xpsD (725-1307) in pCR2.1-TOPO; Kanr | This study |