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RT-PCR of Arabidopsis T-DNA insertion and antisense mutants in AtCOPIA4. Primers used for AtCOPIA4 are P5 and P6, P1 and P6 for the chimeric transcript as shown in Figure 1 and P3 and P4 for RPP4. Total RNA from seedlings was used. Lines used are: Col-0-Columbia wild type; I3, I10-heterozygous and homozygous T-DNA insertion lines, respectively; A60, and A80 are antisense lines. Chimera indicates RPP4- AtCOPIA4 chimeric mRNA.
