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. 2010 Mar 1;33(1):36–43. doi: 10.1590/S1415-47572010005000015

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Copyright © 2010, Sociedade Brasileira de Genética.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Sequence variations among partial sequences of lgbp, and the development of an indel marker. a. The multi-alignment of sequences amplified from BAC plasmids (LGBP-BAC1 and LGBP-BAC2) and genomic DNA (LGBP-genomic1 and LGBP-genomic2), showing insertion-deletion variation and several nucleotide mutations. b. Organization of the two variants; the numbers stand for fragment length (bp). The location of three primers (CFLGBPF1, CFLGBPR2 and CFLGBPR4) is indicated by triangles. c. Indel marker testing by using 13 randomly selected DNA samples showed the PCR products of different variants successfully separated from each other. A mixture of the two variants was used as positive controls (Lane PC).