Table 1.
Spontaneous and induced frequencies of BNMN and MNMN in lymphocyte cultures treated in vitro with different concentrations of AZT, 3TC and d4T.
| Donor 1
|
Donor 2
|
Donor 3
|
Average
|
|||||||||||||||
| BNMNc |
MNMNc |
BNMNc |
MNMNc |
BNMNc |
MNMNc |
BNMNd | MNMNd | NDIe | ||||||||||
| Rep 1 | Rep 2 | Rep 1 | Rep 2 | Rep 1 | Rep 2 | Rep 1 | Rep 2 | Rep 1 | Rep 2 | Rep 1 | Rep 2 | |||||||
| AZT | ||||||||||||||||||
| NCa | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 2.00 ± 1.26 | 0.00 ± 0.00 | 2.03 ± 0.16 | |||
| 125 μg/mL | 1 | 2 | 0 | 0 | 1 | 2 | 0 | 0 | 2 | 2 | 0 | 0 | 3.34 ± 1.04 | 0.00 ± 0.00 | 1.90 ± 0.09 | |||
| 250 μg/mL | 3 | 3 | 1 | 0 | 4 | 1 | 0 | 0 | 2 | 2 | 1 | 0 | 5.00 ± 2.10** | 0.66 ± 1.04 | 1.90 ± 0.09 | |||
| 375 μg/mL | 3 | 3 | 0 | 0 | 3 | 3 | 0 | 0 | 3 | 4 | 1 | 0 | 6.34 ± 0.82*** | 0.34 ± 0.82 | 1.82 ± 0.12** | |||
| 500 μg/mL | 5 | 6 | 1 | 0 | 5 | 3 | 1 | 0 | 4 | 5 | 1 | 0 | 9.34 ± 2.26*** | 1.00 ± 1.10 | 1.77 ± 0.08** | |||
| 625 μg/mL | 4 | 5 | 1 | 0 | 5 | 5 | 0 | 1 | 6 | 5 | 1 | 0 | 10.00 ± 1.26*** | 1.00 ± 1.10 | 1.72 ± 0.07*** | |||
| 3TC | ||||||||||||||||||
| NCa | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0.66 ± 1.04 | 0.00 ± 0.00 | 2.02 ± 0.10 | |||
| 125 μg/mL | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 2.34 ± 1.50 | 0.00 ± 0.00 | 1.88 ± 0.75 | |||
| 250 μg/mL | 1 | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 3 | 1 | 0 | 0 | 3.34 ± 1.64** | 0.00 ± 0.00 | 1.83 ± 0.10* | |||
| 500 μg/mL | 2 | 3 | 0 | 0 | 2 | 3 | 0 | 0 | 3 | 2 | 0 | 0 | 5.00 ± 1.10*** | 0.00 ± 0.00 | 1.80 ± 0.06** | |||
| 750 μg/mL | 4 | 3 | 0 | 1 | 3 | 3 | 0 | 0 | 2 | 3 | 0 | 0 | 6.00 ± 1.26*** | 0.34 ± 0.82 | 1.73 ± 0.10*** | |||
| d4T | ||||||||||||||||||
| NCa | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1.66 ± 1.96 | 1.00 ± 1.10 | 2.08 ± 0.15 | |||
| 125 μg/mL | 6 | 5 | 3 | 3 | 3 | 5 | 4 | 3 | 3 | 8 | 4 | 2 | 10.00 ± 3.80*** | 6.34 ± 1.50*** | 1.53 ± 0.08*** | |||
| 250 μg/mL | 3 | 4 | 2 | 1 | 3 | 5 | 1 | 2 | 4 | 4 | 0 | 1 | 7.66 ± 1.50*** | 2.34 ± 1.50 | 1.47 ± 0.10*** | |||
| 500 μg/mL | 4 | 4 | 3 | 1 | 2 | 3 | 1 | 3 | 2 | 2 | 0 | 0 | 5.66 ± 1.96** | 2.66 ± 2.74 | 1.47 ± 0.12*** | |||
| 750 μg/mL | 2 | 4 | 1 | 2 | 4 | 2 | 1 | 0 | 2 | 4 | 2 | 1 | 6.00 ± 2.18** | 2.34 ± 1.50 | 1.33 ± 0.08*** | |||
| PCb | ||||||||||||||||||
| 6 μg/mL | 16 | 19 | 0 | 1 | 23 | 18 | 1 | 1 | 20 | 18 | 0 | 1 | 37.66 ± 4.80 | 1.34 ± 1.04 | 1.80 ± 0.14 | |||
aNC: negative control; bPC: positive control (bleomycin). cMNMN: mononucleated micronucleated cells; BNMN: binucleated micronucleated cells. For each replicate (Rep), 500 binucleates were scored to give a total of 1,000 binucleated cells (i.e. 2,000 nuclei) for each donor. No cells had more than one micronucleus. dTotal number of micronuceli in 1,000 binucleates ± standard deviation (SD). eNDI: nuclear division index. *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001 compared to the corresponding negative controls.