Skip to main content
. Author manuscript; available in PMC: 2012 Jan 15.
Published in final edited form as: Dev Biol. 2010 Nov 10;349(2):483–493. doi: 10.1016/j.ydbio.2010.11.006

Fig. 1. alcama morphants have defects in facial skeletal patterning.

Fig. 1

(A) Graphic representation of alcama RNA illustrating the position of TB and SB MOs. (B) Nomarski image of a 48 hpf WT larva with the box indicating the region shown in (C-E). 48 hpf Control (C), alcama TB (D), and alcama SB (E) morphants stained with Zn-5 antibody. Both MOs effectively knock down alcama expression in the heart (arrowheads) and pouches (arrows). (F, G) Lateral views of 5 dpf larvae injected with control and alcama TB MOs. alcama morphants have a protruding jaw (arrows), cardiac edema (arrowheads) and an absent swim bladder (open arrows). Lateral views of Alcian blue stained Control (H) and alcama (I) morphants at 5 dpf. The line indicates the length of the pharyngeal cartilage, which is shortened in alcama morphants. The arrow points to Meckels cartilage, which is bent ventrally in alcama morphants. Scale bars: 50 μm.