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. Author manuscript; available in PMC: 2012 Jan 15.
Published in final edited form as: Dev Biol. 2010 Nov 10;349(2):483–493. doi: 10.1016/j.ydbio.2010.11.006

Fig. 5. alcama rescues NC differentiation defect in edn1-/- mutants.

Fig. 5

Lateral views of 30 hpf larvae with representative absent (A), partial (B) or strong (C) staining for dlx5a. Bar graphs show the percentage dlx5a expressing larvae in edn1-/- mutants (D) and WT (E) following the stated injections. edn1 or alcama RNA was injected with or without alcama MO. alcama RNA decreases the percentage of affected individuals (absent dlx5a staining) by nearly 30% in edn1-/- mutants and co-injection of alcam Mo abrogates the ability of edn1 RNA to rescue dlx5a expression in edn1-/- mutants. Indicated p-values are calculated by Fishers exact test, n depicts the number of experiments and N depicts the total number of larvae represented in the columns of the plot (p-value < 0.0001 for both D and E by χ2 analysis). a1 and a2 label pharyngeal arches 1 and 2. Dotted lines highlight pharyngeal pouches 1 and 2. Scale bar: 50 μm.