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. Author manuscript; available in PMC: 2012 Jan 15.
Published in final edited form as: Dev Biol. 2010 Nov 10;349(2):483–493. doi: 10.1016/j.ydbio.2010.11.006

Fig. 6. nadl1.1 morphants phenocopy alcama morphant cartilage defects.

Fig. 6

Dorsal views (A-C) and lateral views (D, E) of 30 hpf larvae. nadl1.1 is expressed in the pharyngeal arches, diencephalon, telencephalon, hindbrain neurons, neural tube and pectoral fin in 30 hpf WT larvae (A). 30 hpf nadl 1.1 morphants have down-regulated hand2 (C) and dlx5a (E) expression as compared to uninjected WT (B, D). (F) Bar graph showing that the percentage of larvae with down-regulated (absent) dlx5a expression remains unchanged in the p53-/- background (p-value = 1.000 by Fishers exact test, indicating that WT and p53-/- mutants are not significantly different). Dissected mandibular and hyoid elements from 5 dpf WT and nadl1.1 morphant larvae stained with Alcian blue (G, H) and their corresponding schematics (G′, H′). The joint fusions in nadl1.1 morphants (H, H′) marked by * resemble those seen in alcama morphants. (I) Bar graph comparing the percentage of larvae with affected cartilage in alcama morphants in WT versus p53-/-mutants (p-value = 0.1941 by Fishers exact test, indicating that WT and p53-/- mutants are not significantly different). N is the total number of larvae from three experiments. Scale bars: 50 μm.