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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Eur J Neurosci. 2010 Mar 19;31(7):1219–1232. doi: 10.1111/j.1460-9568.2010.07156.x

Figure 10. Qualitative Western blots reveal the expression of canonical transient receptor proteins 3 and 7 (TRPC3 and TRPC7) in the VRG.

Figure 10

A) ʃVRG activity recorded from the whole medullary slice, in control conditions (ACSF). B) ʃVRG activity recorded from the VRG-island after its isolation from the rest of the medullary slice, in control conditions (ACSF). C) NK1 receptors (10μg total protein/ lanes 1-3); and, D) TRPC1 channels are expressed in the cortex, but not in the cerebellum or VRG-island preparations, 15μg total protein/ lanes). TRPC3 channels are expressed in all the VRG-islands tested (15μg total protein/ lanes 1-3). TRPC4 channels are expressed in the cortex and cerebellum but not the VRG-islands, (15μg total protein/ lanes), whereas TRPC5 channels (15μg total protein/ lanes) and TRPC6 channels preparations, 15μg total protein/ lanes) are expressed in the cortex and, but not in the cerebellum or VRG-islands. TRPC7 channels are expressed in the cerebellum and VRG-islands (15μg total protein/ lanes). E) TRPM4 channels were detected in the cortex (10μg total protein/ lanes 1 and 2) and cerebellum (10μg total protein/ lanes 1 and 2) but does not appear to be expressed in the VRG-Island (VRG-Island preparations; 10μg total protein/ lanes 1 and 3; 50μg total protein/lanes 2 and 4).