Table 2. Influence of spiking on the performance of viral nucleic acid purification.
Virus | Unspiked specimen (A) | IC spiked specimen (B) | Wilcoxon test | |
a | CMV 10−3 | 33.6 | 33.8 | NS |
CMV 10−4 | 36.3 | 36.6 | NS | |
CMV 10−5 | 39.6 | 39.4 | NS | |
b | EV 10−3 | 30.1 | 30.6 | NS |
EV 10−4 | 33.7 | 33.8 | NS | |
EV 10−5 | 38.2 | 37.8 | NS |
Cycles Thresholds (Ct) of serial dilutions of supernatant media of CMV (a) or EV (b) cell cultures unspiked (A) or spiked (B) with T4-MS2 IC were presented here. Each samples were tested in triplicate (the mean value was calculated). The Wilcoxon test were used to compared Ct values (NS = non significative).