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Wet laboratory validation of in silico identified genic-SSR length polymorphism between pigeonpea parental lines. Pigeonpea genic-SSR locus ASSR-8 showing: a. in silco polymorphism between the aligned TSA contigs of parental lines Asha (A) and UPAS 120 (U), b. agarose gel analysis of segregation of the ASSR-8 alleles in F2 population. Positions of flanking primers and the polymorphic SSR sequence are highlighted.
