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. 2011 Jan 20;12:50. doi: 10.1186/1471-2164-12-50

Table 3.

Model of RNAi reagent disambiguation methods under one, two or three reagents per gene.

1 RNAi Reagent/Gene 2 RNAi Reagents/Gene 3 RNAi Reagents/Gene
"Lenient" Rule A: Number of False Negatives RFN × H RFN2×H RFN3×H

"Lenient" Rule A: Number of False Positives RFP × N (RFP×2-RFP2)×N (RFP×3-RFP2×3+RFP3)×N

"Stringent" Rule B: Number of False Negatives RFN × H (RFN×2-RFN2)×H (RFN×3-RFN2×3+RFN3)×H

"Stringent" Rule B: Number of False Positives RFP × N RFP2×N RFP3×N

"Balanced" Rule C: Number of False Negatives RFN × H (RFN×2-RFN2)×H (RFN2×3-RFN3×2)×H

"Balanced" Rule C: Number of False Positives RFP × N RFP2×N (RFP2×3-RFP3×2)×N

N = The number of genes in the screening library, H = The number of genes a screen should uncover under ideal conditions, RFN = Fraction of reagents of H that fail. RFP = Fraction of reagents of N that are false positives. Rule "A": Only one reagent targeting a gene need to be a "hit" for the gene to be called a "hit". Rule "B": All reagents targeting a gene need to be a "hit". Rule "C": More than half of reagents must score as a "hit".

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