Table 3.
Model of RNAi reagent disambiguation methods under one, two or three reagents per gene.
1 RNAi Reagent/Gene | 2 RNAi Reagents/Gene | 3 RNAi Reagents/Gene | |
---|---|---|---|
"Lenient" Rule A: Number of False Negatives | RFN × H | ||
"Lenient" Rule A: Number of False Positives | RFP × N | ||
"Stringent" Rule B: Number of False Negatives | RFN × H | ||
"Stringent" Rule B: Number of False Positives | RFP × N | ||
"Balanced" Rule C: Number of False Negatives | RFN × H | ||
"Balanced" Rule C: Number of False Positives | RFP × N |
N = The number of genes in the screening library, H = The number of genes a screen should uncover under ideal conditions, RFN = Fraction of reagents of H that fail. RFP = Fraction of reagents of N that are false positives. Rule "A": Only one reagent targeting a gene need to be a "hit" for the gene to be called a "hit". Rule "B": All reagents targeting a gene need to be a "hit". Rule "C": More than half of reagents must score as a "hit".