Table 3.
Evolutionary highly conserved motifs in Influenza A virus
| Segment | Motif | Length | start position (consensus sequence) | End Position (Consensus Sequence) | H5N1 Start Position | H5N1 End Position | Sequence | Repeated Positions On H5N1 | Mapping on Conserved Regions |
|---|---|---|---|---|---|---|---|---|---|
| 1 (PB2) | 1 | 9 | 1652 | 1660 | 1631 | 1639 | TGATGTGGG | R8 | |
| 1 (PB2) | 2 | 6 | 2254 | 2259 | 2232 | 2237 | GAAACG | (2238 - 2243) | R12 |
| 1 (PB2) | 3 | 11 | 2273 | 2283 | 2251 | 2261 | AGCATACTTAC | R12 | |
| 1 (PB2) | 4 | 9 | 2291 | 2299 | 2269 | 2277 | CAGACAGCG | R12 | |
| 1 (PB2) | 1 | 6 | 914 | 919 | 889 | 894 | ATGATG | (1243 - 1248) (1246 - 1251) (1624 - 1629) (1994 - 1999) |
R8 |
| 2 (PB1) | 2 | 11 | 1268 | 1278 | 1243 | 1253 | ATGATGATGGG | R10 | |
| 2 (PB1) | 3 | 6 | 2354 | 2359 | 2248 | 2253 | GAGATC | (101 to 106) | R23, R25 |
| 2 (PB1) | 4 | 6 | 2391 | 2396 | 2285 | 2290 | GACGGC | R24, R25 | |
| 3 (PA) | 1 | 8 | 2140 | 2147 | 2095 | 2102 | GAGGAGTG | R18 | |
| 3 (PA) | 2 | 6 | 2150 | 2155 | 2105 | 2110 | TGATTA | R18 | |
| 7 (M) | 1 | 9 | 93 | 101 | 77 | 85 | GGCCCCCTC | R1 | |
| 7 (M) | 2 | 11 | 180 | 190 | 164 | 174 | AAGACAAGACC | R1 | |
| 7 (M) | 3 | 8 | 196 | 203 | 180 | 187 | TGTCACCT | R1 | |
| 7(M) | 4 | 17 | 237 | 253 | 221 | 237 | CTCACCGTGCCCAGTGA | R2 | |
| 7 (M) | 5 | 7 | 873 | 879 | 855 | 861 | TTCAAAT | R8 | |
| 7 (M) | 6 | 8 | 945 | 952 | 927 | 934 | ATGAGGGA | R8 | |
| 8 (NS) | 1 | 7 | 69 | 75 | 43 | 49 | AGGTAGA | R1 | |
| 8 (NS) | 2 | 10 | 574 | 583 | 529 | 538 | AGGATGTCAA | R6 | |
| 8 (NS) | 3 | 6 | 616 | 621 | 571 | 576 | AATGGA | ( 368- 373) (383- 388) |
R6 |
The evolutionary conserved motifs found in four of the eight influenza segments, whose criteria are at least 6 consecutive nucleotides that are 100% conserved in all the studied strains and the location of each motif on H5N1 Refseq. Sequences and length are shown