Figure 5. Density of doublecortin-positive fibers in CA3 hippocampal region of control and APP_Sw,Ind mice.

(A) Representative epifluorescence microscopy images of the stratum lucidum stained for doublecortin (Dcx, red) and Hoechst (blue). Scale bar  = 50 µm. (B). Semiquantitative analysis reveals a significant reduction in the area ocuppied by Dcx-positive fibers in the stratum lucidum of the CA3 region in APP_Sw,Ind mice. Enrichment increased significantly the density of Dcx staining in the stratum lucidum in both WT and APP_Sw,Ind mice compared to standard housed mice. Data represent mean ± SEM. *p<0.01, compared to WT mice. ^# p<0.01, compared to APP_Sw,Ind mice. Number of mice/group: 6.